Please bear in mind that you can save this formula by using the 'Save' button. Finally, follow the menu 'Processing/Arithmetics' and enter the following formula: IMPORTANT: Remember that even if only the active spectrum is visible, unless the “Apply Processing to All spectra in Stack” option is off, all spectra in the stack will also be processed. We are going to correct slightly the phase of the first slice (the phase of the other slices will be automatically corrected): Should you want to move to another spectrum without resorting to the stack mode just press Shift+Mouse Wheel or Shift + Up/Down keys. While working on this mode, you will see on the screen just the active spectrum (the spectrum which is highlighted in green in the stacked plot). We recommend you to select 'Active spectrum' in the sixth button of the left-vertical toolbar:
Otherwise you will need to apply a phase correction. When you load this kind of spectra into Mnova, you will obtain something like this:Īs you can see, you will have in the first slice (red slice with final pulse: 45 degrees) all the protonated signals, in the second and third slices you will get only the CH signals up and in the forth slice (purple slice with final pulse of 135 degrees) a spectrum with the CH and CH3 up and the CH2 down Then, please make sure that the phase of all the slices is right. As you well know, the DEPT pulse program in Varian generates a stacked plot with four slices, acquired with phase of the last pulse equal to 45, 90, 90, 135 degrees.
This tutorial shows you how to process a Varian DEPT spectrum using Mnova software.
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